User:Tara K. Luckau/Notebook/Team ConGen/2012/11/27: Difference between revisions
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====MP1a==== | ====MP1a==== | ||
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{| {{table}} style="text-align: center" | |||
: still fine, though seems to decrease with increasing Acos5 | |- | ||
= | ! !! 23 July 2012 !! this time | ||
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| style="background: blue" | Ai5043 || did not amplify || did not amplify; '''remove from multiplex''' | |||
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| style="background: green" | Acos3 || amplified well; no changes needed || still fine, though seems to decrease with increasing Acos5 & Ai5071 | |||
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= | | style="background: yellow" | Acos5 || amplified with high intensity, pull down a bit || 0.04 = too low; 0.06 = inconclusive (failures); 0.08 = too high | ||
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| style="background: yellow" | Ai5013 || amplified with very low intensity; increase concentration || second peak never got above 250, regardless of concentration; but in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased | |||
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| style="background: red" | Ai5071 || amplified with lower intensity; increase a bit || best at 0.24, 0.28, 0.32 (200-300 intensity only) | |||
= | |} | ||
====MP1b==== | ====MP1b==== | ||
{| {{table}} style="text-align: center" | |||
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! !! 23 July 2012 !! this time | |||
|- | |||
| style="background: blue" | Ai5033 || amplified well; no changes needed || still fine (no amp for any markers in Ai5057 assay) | |||
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| style="background: blue" | Ai5057 || did not amplify || did not amplify; '''remove from multiplex''' | |||
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| style="background: green" | Ai5037 || amplified well; no changes needed || still fine (no amp for any markers in Ai5057 assay) | |||
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| style="background: yellow" | Ai5035 || amplified well; no changes needed || a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay) | |||
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| style="background: red" | Ai5062 || amplified but with very low intensity; increase concentration || best at 0.48 (400 & 6000 intensity) | |||
|} | |||
Revision as of 16:02, 27 November 2012
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CNHY MP1a/b Optimization
Frag Results
MP1a
MP1b
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