User:Tara K. Luckau/Notebook/Team ConGen/2012/11/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/11/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
No edit summary
Line 9: Line 9:




==CNTI Acos5==
==SCOC Batch03==
* Samples = USGS TP & CPN + Clark TP & SYR




===PCR===
===Gel===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20121108_Gel.png|800 px]]
 
 
* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
 
 
===Fragment Analysis Submission===
 


* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y


===Gel===


{| {{table}} style="text-align: center"
:: [[Image:20121108_Frag.png|900 px]]
|-
 
! Pour !! Load !! Run
 
|-
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184828 801124184828]
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
:: [[Image:20121108_FedEx.png|700 px]]
|-
 
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


* UAGC Submission# TZNF & WKAY


[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* FedEx picked up 2:48pm, 8 November
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* FedEx delivered 9:16am, 9 November
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* UAGC received 10:55am, 9 November
* UAGC completed 1:54pm, 9 November




Revision as of 16:21, 15 November 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC Batch03

  • Samples = USGS TP & CPN + Clark TP & SYR


Gel


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.


Fragment Analysis Submission

  • 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y




  • UAGC Submission# TZNF & WKAY


  • FedEx picked up 2:48pm, 8 November
  • FedEx delivered 9:16am, 9 November
  • UAGC received 10:55am, 9 November
  • UAGC completed 1:54pm, 9 November




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2012/11/15&oldid=657011"