User:Tara K. Luckau/Notebook/Team ConGen/2012/11/14

Current revision

Tara's Lab Notebook

optimize for marker peak height - vary each primer (based on results from 23 July 2012)
ASHY MP1a: increase Ai5013, Ai5043, Ai5071
ASHY MP1a: decrease Acos5
ASHY MP1b: increase Ai5057, Ai5062

@@ Line 9: / Line 9: @@
-==CNTI Acos5==
+==ASHY MP1a/b Optimization==
+* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
+* ASHY MP1a: increase Ai5013, Ai5043, Ai5071
+* ASHY MP1a: decrease Acos5
+* ASHY MP1b: increase Ai5057, Ai5062
 ===PCR===
-* [[Image:20120110_PCRa.png|800 px]]
+====ASHY MP1a: increase Ai5013, Ai5043, Ai5071====
+:: [[Image:20121114_PCRa.png|800 px]]
-===Gel===
+====ASHY MP1a: decrease Acos5====
+:: [[Image:20121114_PCRb.png|800 px]]
-{| {{table}} style="text-align: center"
-|-
-! Pour !! Load !! Run
-|-
-| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
-|-
-| 27 µL GelRed || 6 µL ladder || 45 minutes
-|}
-[[Image:20120110_Gela.png|800 px]]
-* mispriming everywhere - primer pair not usable for CNHY
-* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
-* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+====ASHY MP1b: increase Ai5057, Ai5062====
+:: [[Image:20121114_PCRc.png|800 px]]