User:Tara K. Luckau/Notebook/Team ConGen/2012/11/08: Difference between revisions

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==CNTI Acos5==
==SCOC Batch02==
 
* Samples = USGS SYR & LOM + Clark HOL & RAJ
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
===Gel===
* [[Image:20121025_Gel.png|800 px]]


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 13:21, 14 November 2012

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SCOC Batch02

  • Samples = USGS SYR & LOM + Clark HOL & RAJ


Gel


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.




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