User:Tara K. Luckau/Notebook/Team ConGen/2012/11/08

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(Autocreate 2012/11/08 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNTI Acos5==
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==SCOC Batch02==
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* Samples = USGS SYR & LOM + Clark HOL & RAJ
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===PCR===
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* [[Image:20120110_PCRa.png|800 px]]
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===Gel===
===Gel===
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* [[Image:20121025_Gel.png|800 px]]
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{| {{table}} style="text-align: center"
 
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|-
 
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! Pour !! Load !! Run
 
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
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| 27 µL GelRed || 6 µL ladder || 45 minutes
 
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|}
 
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[[Image:20120110_Gela.png|800 px]]
 
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* mispriming everywhere - primer pair not usable for CNHY
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* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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Revision as of 16:21, 14 November 2012

Tara's Lab Notebook Main project page
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Contents


SCOC Batch02

  • Samples = USGS SYR & LOM + Clark HOL & RAJ


Gel


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.




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