User:Tara K. Luckau/Notebook/Team ConGen/2012/11/06

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/11/06 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Line 9: Line 9:
-
==CNTI Acos5==
+
===SCOC Batch03==
 +
* Samples = USGS TP & CPN + Clark TP & SYR
===PCR===
===PCR===
-
* [[Image:20120110_PCRa.png|800 px]]
+
* MP1-B
-
 
+
* [[Image:20121106_PCR.png|800 px]]
-
 
+
-
===Gel===
+
-
 
+
-
{| {{table}} style="text-align: center"
+
-
|-
+
-
! Pour !! Load !! Run
+
-
|-
+
-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
+
-
|-
+
-
| 27 µL GelRed || 6 µL ladder || 45 minutes
+
-
|}
+
-
 
+
-
 
+
-
[[Image:20120110_Gela.png|800 px]]
+
-
 
+
-
* mispriming everywhere - primer pair not usable for CNHY
+
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
 +
* [[Image:20121105_Grid.png|800 px]]

Revision as of 16:19, 14 November 2012

Tara's Lab Notebook Main project page
Previous entry      Next entry

Contents


=SCOC Batch03

  • Samples = USGS TP & CPN + Clark TP & SYR


PCR

  • MP1-B




Retrieved from "http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/11/06"
Personal tools