User:Tara K. Luckau/Notebook/Team ConGen/2012/10/29: Difference between revisions

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==CNTI Acos5==
==SCOC Batch02==




===PCR===
===Fragment Analysis Submission===
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


* [[Image:20121029_Frag.png|900 px]]


[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=876927708843 876927708843]
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
:: [[Image:20121029_FedEx.png|700 px]]
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




* UAGC Submission# QMCC & SYAZ
* FedEx picked up ????pm, 29 Octover
* FedEx delivered ????am, 30 October
* UAGC received ????am, 30 October
* UAGC completed ????am, 31 October




Revision as of 09:42, 26 October 2012

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SCOC Batch02

Fragment Analysis Submission

  • 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y




  • UAGC Submission# QMCC & SYAZ


  • FedEx picked up ????pm, 29 Octover
  • FedEx delivered ????am, 30 October
  • UAGC received ????am, 30 October
  • UAGC completed ????am, 31 October



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