User:Tara K. Luckau/Notebook/Team ConGen/2012/10/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2012/10/25 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
No edit summary
Line 9: Line 9:




==CNTI Acos5==
==SCOC Batch 2==
 
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]




Revision as of 15:19, 25 October 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC Batch 2

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2012/10/25&oldid=642013"