User:Tara K. Luckau/Notebook/Team ConGen/2012/10/25

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(Autocreate 2012/10/25 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNTI Acos5==
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==SCOC Batch 2==
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===PCR===
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* [[Image:20120110_PCRa.png|800 px]]
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Revision as of 17:19, 25 October 2012

Tara's Lab Notebook Main project page
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Contents


SCOC Batch 2

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by




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