User:Tara K. Luckau/Notebook/Team ConGen/2012/08/02: Difference between revisions

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==CNTI Acos5==
==Field Work==




===PCR===
* July 30 - August 2: SYR
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
===SYR Tissue Count - this week===
 


{| {{table}} style="text-align: center"
{| {{table}} style="text-align: center"
|-
|-
! Pour !! Load !! Run
! !! A <br /><small>8, 32, 33</small> !! B <br /><small>9, 10, 29, 11, 25</small> !! C <br /><small>13, 14</small>
|-
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
| CNTI || 0 || 0 || 0
|-
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
| SCOC || 0 || 0 || 0
|}
|}




[[Image:20120110_Gela.png|800 px]]
===SYR Tissue Count - New Totals===
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




{| {{table}} style="text-align: center"
|-
! !! A <br /><small>8, 32, 33</small> !! B <br /><small>9, 10, 29, 11, 25</small> !! C <br /><small>13, 14</small>
|-
| CNTI || style="background: orange" | 24 || style="background: Chartreuse" | 36 || style="background: red" | 10
|-
| SCOC || style="background: Chartreuse" | 36 || style="background: Chartreuse" | 42 || style="background: orange" | 24
|}




Revision as of 15:57, 2 August 2012

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Field Work

  • July 30 - August 2: SYR


SYR Tissue Count - this week

A
8, 32, 33		 B
9, 10, 29, 11, 25		 C
13, 14
CNTI 0 0 0
SCOC 0 0 0


SYR Tissue Count - New Totals

A
8, 32, 33		 B
9, 10, 29, 11, 25		 C
13, 14
CNTI 24 36 10
SCOC 36 42 24



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