User:Tara K. Luckau/Notebook/Team ConGen/2012/07/20: Difference between revisions

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==CNTI Acos5==
==Field Work==




===PCR===
* July 16-20: RAJ/HOL
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
===RAJ/HOL Tissue Count - this week===
 


{| {{table}} style="text-align: center"
{| {{table}} style="text-align: center"
|-
|-
! Pour !! Load !! Run
! !! A <br /><small>HOL 5, 6, 7</small> !! B <br /><small>RAJ 9, 10</small> !! C <br /><small>RAJ 19, 20</small>
|-
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
| CNHY || ? || 0 || ?
|-
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
| SCOC || ? || 0 || ?
|}
|}




[[Image:20120110_Gela.png|800 px]]
===RAJ/HOL Tissue Count - New Totals===
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




{| {{table}} style="text-align: center"
|-
! !! A <br /><small>HOL 5, 6, 7</small> !! B <br /><small>RAJ 9, 10</small> !! C <br /><small>RAJ 19, 20</small>
|-
| CNHY || style="background: orange" | 21 || style="background: Chartreuse" | 56 || style="background: orange" | 28
|-
| SCOC || style="background: Chartreuse" | 36 || style="background: Chartreuse" | 44 || style="background: orange" | 22
|}




Revision as of 17:58, 19 July 2012

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Field Work

  • July 16-20: RAJ/HOL


RAJ/HOL Tissue Count - this week

A
HOL 5, 6, 7		 B
RAJ 9, 10		 C
RAJ 19, 20
CNHY ? 0 ?
SCOC ? 0 ?


RAJ/HOL Tissue Count - New Totals

A
HOL 5, 6, 7		 B
RAJ 9, 10		 C
RAJ 19, 20
CNHY 21 56 28
SCOC 36 44 22



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