User:Tara K. Luckau/Notebook/Team ConGen/2012/07/17: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 28: Line 28:
* Primer pairs grouped into the two multiplexes based on primer interactions, annealing temperature, and amplicon size
* Primer pairs grouped into the two multiplexes based on primer interactions, annealing temperature, and amplicon size
* all primers at same concentration
* all primers at same concentration
* GOAL: just to see  
* GOAL: to see whether all loci are amplifying and at what intensity




[[Image:20120717_PCRc.png|800 px]]
[[Image:20120717_PCRc.png|800 px]]
[[Image:20120717_PCRd.png|800 px]]




[[Image:20120717_PCRd.png|800 px]]





Revision as of 15:38, 18 July 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC MP1

PCR MP1-G+Y

  • try to combine MP1-G with MP1-Y (same buffer and annealing temperature, limited primer interactions)


CNHY/TI MP1

PCR Uniplex Ai5033

  • Uniplex


PCR MP1a and MP1b

  • Primer pairs grouped into the two multiplexes based on primer interactions, annealing temperature, and amplicon size
  • all primers at same concentration
  • GOAL: to see whether all loci are amplifying and at what intensity