User:Tara K. Luckau/Notebook/Team ConGen/2012/07/17: Difference between revisions

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==CNTI Acos5==
==SCOC MP1==




===PCR===
===PCR MP1-G+Y===
* [[Image:20120110_PCRa.png|800 px]]
* try to combine MP1-G with MP1-Y (same buffer and annealing temperature, limited primer interactions)
* [[Image:20120717_PCRa.png|800 px]]




===Gel===
==CNHY/TI MP1==
===PCR Uniplex Ai5033===
* Uniplex
[[Image:20120717_PCRb.png|800 px]]


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


[[Image:20120110_Gela.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 15:03, 17 July 2012

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SCOC MP1

PCR MP1-G+Y

  • try to combine MP1-G with MP1-Y (same buffer and annealing temperature, limited primer interactions)


CNHY/TI MP1

PCR Uniplex Ai5033

  • Uniplex





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