User:Tara K. Luckau/Notebook/Team ConGen/2012/07/15: Difference between revisions

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(Autocreate 2012/07/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
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==CNTI Acos5==
==SCOC MP1-G==




===PCR===
decreasing gradient of Scun15 concentration (peak too high) (Scun9 and Scun19 concentrations held constant)
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
===Fragment Analysis Results===


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


* TP sample looks poopy for all conditions ... possible degraded sample; do not evaluate.
* SYR sample is of good quality ... condition where '''Scun15 is at 0.1µL / reaction''' is best


[[Image:20120110_Gela.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]


* [[Image:20120715_FragResults1.png|600 px]]
* [[Image:20120715_FragResults2.png|600 px]]
* [[Image:20120715_FragResults3.png|600 px]]
* [[Image:20120715_FragResults4.png|600 px]]
* [[Image:20120715_FragResults5.png|600 px]]
* [[Image:20120715_FragResults6.png|600 px]]




Revision as of 17:46, 15 July 2012

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SCOC MP1-G

decreasing gradient of Scun15 concentration (peak too high) (Scun9 and Scun19 concentrations held constant)


Fragment Analysis Results

  • TP sample looks poopy for all conditions ... possible degraded sample; do not evaluate.
  • SYR sample is of good quality ... condition where Scun15 is at 0.1µL / reaction is best




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