User:Tara K. Luckau/Notebook/Team ConGen/2012/06/11: Difference between revisions

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==CNTI Acos5==
==Summer 2012 Plans==




===PCR===
===Field Work===
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
====SYR====
 
 
{| {{table}} style="text-align: center"
|-
! !! A !! B !! C
|-
| CNTI || 24 || 36 || 9
|-
| SCOC || 36 || 42 || 21
|}
 
 
====RAJ/HOL====
 
 
{| {{table}} style="text-align: center"
|-
! !! A !! B !! C
|-
| CNHY || 13 || 56 || 27
|-
| SCOC || 36 || 44 || 21
|}
 
 
====TP====
 
 
{| {{table}} style="text-align: center"
|-
! !! A !! B !! C
|-
| CNHY || 1 || 30 || 19
|-
| SCOC || 28 || 40 || 19
|}
 
 
====LOM====
 


{| {{table}} style="text-align: center"
{| {{table}} style="text-align: center"
|-
|-
! Pour !! Load !! Run
! !! A !! B !! C
|-
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
| CNHY || 1 || || 5
|-
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
| SCOC || 15 || 13 || 22
|}
|}




[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
===Lab Work===
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
 
 
*  




Revision as of 19:17, 11 June 2012

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Summer 2012 Plans

Field Work

SYR

A B C
CNTI 24 36 9
SCOC 36 42 21


RAJ/HOL

A B C
CNHY 13 56 27
SCOC 36 44 21


TP

A B C
CNHY 1 30 19
SCOC 28 40 19


LOM

A B C
CNHY 1 5
SCOC 15 13 22


Lab Work




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