User:Tara K. Luckau/Notebook/Team ConGen/2012/03/30: Difference between revisions

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throw it all together to see what happens
throw it all together to see what happens
::this is by far the best sample: many markers seemed to amplify
::this is by far the best sample: many markers seemed to amplify (Ai5033, Ai5037, Acos5, Ai5035, Ai5062), only Ai5057's amplicon is not seen
::all other samples only amplified Acos5
::all other samples only amplified Acos5
::[[Image:20120330_MPA.png|1000 px]]
::[[Image:20120330_MPA.png|900 px]]




Revision as of 14:11, 30 March 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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SCOC Multiplex Optimization

Fragment Analysis Results

SCOC MPY Uniplexes

Scun3


Scun11


sar80


SCOC MPG

for evenness

this is the best sample; most had extremely high Scun15 and extremely low Scun19, low Scun9


+ sar80

sar80 did not amplify at all when multiplexed with the green lane


SCOC Conclusions

  • Scun11 range is very large and overlaps with sar80
  • sar80 seems non polymorphic?
  • because of sar80's resistance to amplify and overlapping amplicon range with Scun11, needs to be run separately from the other 9 markers for SCOCs
  • may be able to integrate it with the 8 SW markers not yet screened


CNHY Multiplex Optimization

Fragment Analysis Results

CNHY MPA

throw it all together to see what happens

this is by far the best sample: many markers seemed to amplify (Ai5033, Ai5037, Acos5, Ai5035, Ai5062), only Ai5057's amplicon is not seen
all other samples only amplified Acos5


CNHY Conclusions

  • oye, lots of work still to do, here




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