User:Tara K. Luckau/Notebook/Team ConGen/2011/09/30
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< User:Tara K. Luckau | Notebook | Team ConGen | 2011 | 09(Difference between revisions)
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Current revision (14:28, 3 October 2011) (view source) (Undo revision 543636 by Redirect fixer (Talk)) |
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| - | # | + | {|{{table}} width="800" |
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| + | |style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span> | ||
| + | |style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
| + | |- | ||
| + | | colspan="2"| | ||
| + | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
| + | __TOC__ | ||
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| + | |||
| + | ==sar84== | ||
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| + | ===Gel=== | ||
| + | |||
| + | {| {{table}} style="text-align: center" | ||
| + | |- | ||
| + | ! Pour !! Load !! Run | ||
| + | |- | ||
| + | | 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | ||
| + | |- | ||
| + | | 27 µL GelRed || 6 µL ladder || 45 minutes | ||
| + | |} | ||
| + | |||
| + | |||
| + | [[Image:20110930_Gela.png|800 px]] | ||
| + | |||
| + | * mispriming everywhere - primer pair not usable for SCOC | ||
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| + | |||
| + | ==SA52.33== | ||
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| + | |||
| + | ===Gel=== | ||
| + | |||
| + | {| {{table}} style="text-align: center" | ||
| + | |- | ||
| + | ! Pour !! Load !! Run | ||
| + | |- | ||
| + | | 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | ||
| + | |- | ||
| + | | 27 µL GelRed || 6 µL ladder || 45 minutes | ||
| + | |} | ||
| + | |||
| + | |||
| + | [[Image:20110930_Gelb.png|800 px]] | ||
| + | |||
| + | * mispriming everywhere - primer pair not usable for SCOC | ||
| + | |||
| + | |||
| + | ==SA60.02== | ||
| + | |||
| + | |||
| + | ===PCR=== | ||
| + | * [[Image:20110930_PCR.png|800 px]] | ||
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| + | |||
| + | |||
| + | |||
| + | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
| + | |} | ||
| + | |||
| + | __NOTOC__ | ||
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sar84Gel
SA52.33Gel
SA60.02PCR
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