User:Tara K. Luckau/Notebook/Team ConGen/2010/10/28

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Gel Scun2 F-R

Cast Gel

  • medium caster: 20cm x 13cm x 0.5cm = 130mL TAE
  • 130mL TAE + 2.6g (2%) agarose in 250mL Erlenmyer flask
  • small stirbar, hotplate set at 200, stir set at 150, for about 15min, until clear
  • let cool (about 5 min)
  • add 13µL GelRed 10,000X, swirl
  • pour into caster, set 36-tooth comb
  • let set (about 10min)

Load Gel

  • 4µL Ladder
  • 4µL Sample w/ loading dye

Run Gel

  • 110V, 2 hrs

Image Gel

  • Nada, zip, zilch ... poopy
  • WTF?




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