User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2011/08/31
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ObjectiveTo determine how pH effects the biomineralization of gold nanoparticles using the biomineralization procedure by Bakshi, et al (doi:10.1021/jp110296y). Description
DataA spectrum of just the buffer was taken, and the absorbance of that spectrum was subtracted from the spectrums of the mixtures taken approximately every half hour: I'd like to see this graph zoomed in to center around what's going on at 550. What I want to know is the following: Is the baseline just increasing, or is there actually a new peak growing in at 550? Matt Hartings 08:39, 6 September 2011 (EDT) This graph shows that the absorbance at 550nm was increasing, so gold nanoparticles were being formed in the reaction mixture as the gold was reduced: A zoomed in version of the spectrum shows that a peak was beginning to form at 550nm instead of just the baseline increasing: Notes
The color of the mixture remained clear the whole time, but the absorbance of the mixture at 550nm did increase over the 187 minutes the mixture was incubated at approximately 80°C. Update 9/7/11: When the mixture was viewed a week later (after being wrapped in aluminum foil and stored at room temperature all week) the color of it was light purple.
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