User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/09/19: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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### Information sheet on [http://www.safety.gatech.edu/chemical/mercury_and_compounds.pdf Hg]
### Information sheet on [http://www.safety.gatech.edu/chemical/mercury_and_compounds.pdf Hg]
### Note: Both metals are volatile. You MUST only work on solutions of these in the hood. If you take solutions of these out of the hood, they must be in sealed containers. Also, any container that has As or Hg in it must be rinsed (and the rinse must go into the waste containers).
### Note: Both metals are volatile. You MUST only work on solutions of these in the hood. If you take solutions of these out of the hood, they must be in sealed containers. Also, any container that has As or Hg in it must be rinsed (and the rinse must go into the waste containers).
(Objective from Dr. Hartings lab notebook [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/09/03 Dr. Hartings])


==Description==
==Description==
For your Hg and As experiments, you need to have three concentrations: 150ppm, 100ppm, and 50ppm.
For your Hg and As experiments, you need to have three concentrations: 150ppm, 100ppm, and 50ppm.


==Calculations==
==Notes and Calculations==


To make 50, 100, and 150 ppm solutions of Mercury and Arsenic, the following calculations were done.
<u>Mercury stock solution</u>
# 0.0908g HgCl2
## (0.0908g HgCl2)*(1mol HgCl2/271.5g HgCl2)*(1mol HgCl2/1mol Hg)*(200.59g Hg/1mol Hg) = 0.0671g Hg
## put in 250mL volumetric
## 268ppm Hg


V<sub>1</sub>*Conc.<sub>1</sub>=V<sub>2</sub>*Conc.<sub>2</sub>
Making 50 mL Hg solutions from Dr. Hartings's 268 ppm [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/09/19|Hg stock solution]]:
*(50 mL)*(50ppm) = (x)*(268ppm)
**x = [(50 mL)*(50 ppm)]/(268ppm) ='''9.32 mL of Hg Stock Solution'''
*(50 mL)*(100ppm) = (x)*(268ppm)
**x = [(50 mL)*(100 ppm)]/(268ppm) ='''18.66 mL of Hg Stock Solution'''
*(50 mL)*(150ppm) = (x)*(268ppm)
**x = [(50 mL)*(150 ppm)]/(268ppm) ='''27.98 mL of Hg Stock Solution'''
Making 50 mL As solutions from 0.0499 M Arsenite solution:
Converting from M to ppm:
(0.0499 mol Arsenite/L Arsenite)*(74.922 g Arsenic/mol of Arsenite)*(1000 mg Arsenic/1 g Arsenic) = 3738.61 mg/L = 3738.61 ppm
*(100 mL)*(50ppm) = (x)*(3738.61ppm)
**x = [(50 mL)*(50 ppm)]/(3738.61ppm) ='''4.00 mL of Arsenite Stock Solution'''
*(100 mL)*(100ppm) = (x)*(3738.61ppm)
**x = [(50 mL)*(100 ppm)]/(3738.61ppm) ='''2.76 mL of Arsenite Stock Solution'''
*(100 mL)*(150ppm) = (x)*(3738.61ppm)
**x = [(50 mL)*(150 ppm)]/(3738.61ppm) ='''1.32 mL of Arsenite Stock Solution'''
Eleni for prepared the Arsenic solutions.


<br>
<u>Table of Solution Preparation</u>
The stock solution for Arsenic was prepared by [[User:Eleni N. Kalivas/Notebook/CHEM-571/2014/09/19|Eleni]].
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''As Stock Solution'''
| align="center" style="background:#f0f0f0;"|'''0.0499M'''
|-
| As stock solution  ||3738.61ppm
|-
| As 150ppm solution ||4mL stock in 100mL
|-
| As 100ppm Solution ||2.76mL stock in 100mL
|-
| As 50ppm solution||1.32mL stock in 100mL
|-
|}
The mercury solutions were made by [[User:Michael S. Bible/Notebook/581/2014/09/19|Michael Bible]]


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Latest revision as of 00:19, 27 September 2017

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Sept 19 - Film Absorbance of Arsenic and Mercury

  • Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad)
  • Group Name : AMT

Objective

  1. Finish analyzing PVA-malachite green data
  2. Start analysis on X-ray
  3. Start making new PVA and PVA clay films
  4. Set up experiments (like the malachite green experiments) to see how well the films absorb arsenic and mercury
    1. Notes: both of these are toxic metals, and we need to understand how to use/work with them.
      1. You MUST read the following before starting in lab today
      2. Information sheet on As
      3. Information sheet on Hg
      4. Note: Both metals are volatile. You MUST only work on solutions of these in the hood. If you take solutions of these out of the hood, they must be in sealed containers. Also, any container that has As or Hg in it must be rinsed (and the rinse must go into the waste containers).

(Objective from Dr. Hartings lab notebook Dr. Hartings)

Description

For your Hg and As experiments, you need to have three concentrations: 150ppm, 100ppm, and 50ppm.

Notes and Calculations

Mercury stock solution

  1. 0.0908g HgCl2
    1. (0.0908g HgCl2)*(1mol HgCl2/271.5g HgCl2)*(1mol HgCl2/1mol Hg)*(200.59g Hg/1mol Hg) = 0.0671g Hg
    2. put in 250mL volumetric
    3. 268ppm Hg



Table of Solution Preparation The stock solution for Arsenic was prepared by Eleni.

As Stock Solution 0.0499M
As stock solution 3738.61ppm
As 150ppm solution 4mL stock in 100mL
As 100ppm Solution 2.76mL stock in 100mL
As 50ppm solution 1.32mL stock in 100mL

The mercury solutions were made by Michael Bible


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