User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/09/03: Difference between revisions
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==Sept | ==Sept 3 -FTIR-DSC== | ||
* Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad) | * Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad) | ||
*Group Name : AMT | *Group Name : AMT |
Revision as of 10:01, 19 September 2014
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Sept 3 -FTIR-DSC
Objective
My group was assigned to use the UV-Vis today, and to use the FTIR, DSC, PXRD on Friday. Note: On Friday Groups A and B will perform the tasks Groups C and D perform on Wednesday and vice versa. Description
1. Take UV Vis spectra for Malachite green concentrations: 0ppm, 0.20ppm, 0.50ppm, 1.1ppm, 1.4ppm, 1.7ppm, and 2.0ppm Malachite Green 2. Find the two absorbance maxima in the spectra. Plot the absorbance value vs concentration at each ppm for each maximum you find. 3. For each absorbance max, determine the slope of your A vs concentration plots. This will give you the extinction coefficient for the wavelengths you are analyzing
1. We will use UV-Vis spectroscopy to determine the amount of Malachite green absorbed into a film 2. Upon determining the amount of Malachite green in the film, we can determine the equilibrium of free Malachite green vs bound malachite green by creating an I.C.E. table. 3. We can also determine the mass of malachite green absorbed per mass of film 4. To properly determine MG concentration, we will need to dilute our samples so that their absorbance is similar to the absorbance of our calibration curve samples. 1. You can see from the intense color of your MG/film solutions that they are much darker (and will absorb more light) than those you used for your calibration curves (except for the 2ppm).
1. Sample prep to be discussed in class 2. Instrument Settings 1. 2o start angle 2. 40o stop angle 3. 1o/second scan speed (this seems too fast, will check on this) 4. 0.5o sampling width
1. We will be using attenuated total reflectance (ATR) mode for our samples 2. Place the film in the sample holder. Execute measurement 3. Save measurement data onto thumb drive
1. Weigh pan and lids 2. Weigh pan, lids and film 3.Log into Differential Scanning Calorimetry software
DataFigure 1. Shows the weights of the pans, lids and films used for DSC |