User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/08/29: Difference between revisions
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Figure 1. Shows the calibration curve for wavelength 617nm. Beer's Law explains that A= Ɛbc, where Ɛ = molar absorptivity in the units of cm<sup>-1</sup>*M<sup>-1</sup>, b = path length, and c = concentration. The molar absorptivity of the malachite green at 617nm is 0.1193. This is identified by translating Beer's Law to the slope of the calibration curve y=mx+b; where y = absorbance (y axis), x = concentration (x axis), and m = molar absorptivity. | Figure 1. Shows the calibration curve for wavelength 617nm. Beer's Law explains that A= Ɛbc, where Ɛ = molar absorptivity in the units of cm<sup>-1</sup>*M<sup>-1</sup>, b = path length, and c = concentration. The molar absorptivity of the malachite green at 617nm is 0.1193. This is identified by translating Beer's Law to the slope of the calibration curve y=mx+b; where y = absorbance (y axis), x = concentration (x axis), and m = molar absorptivity. | ||
[[Image:617 line fit.png]] | [[Image:617 line fit.png]] | ||
Figure 2. Shows the calibration curve for wavelength 423nm. Beer's Law explains that A= Ɛbc, where Ɛ = molar absorptivity in the units of cm<sup>-1</sup>*M<sup>-1</sup>, b = path length, and c = concentration. The molar absorptivity of the malachite green at 617nm is 0.1193. This is identified by translating Beer's Law to the slope of the calibration curve y=mx+b; where y = absorbance (y axis), x = concentration (x axis), and m = molar absorptivity. The standard curve for wavelength 423 has an line fit that is below acceptable values for calibration plots. This is due to the calibration point 1.1. | Figure 2. Shows the calibration curve for wavelength 423nm. Beer's Law explains that A= Ɛbc, where Ɛ = molar absorptivity in the units of cm<sup>-1</sup>*M<sup>-1</sup>, b = path length, and c = concentration. The molar absorptivity of the malachite green at 617nm is 0.1193. This is identified by translating Beer's Law to the slope of the calibration curve y=mx+b; where y = absorbance (y axis), x = concentration (x axis), and m = molar absorptivity. The standard curve for wavelength 423 has an line fit that is below acceptable values for calibration plots. This is due to the calibration point 1.1. | ||
[[Image:Calibration423.png]] | |||
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Aug 29-Completing film synthesis
ObjectiveCompleting film synthesis, starting new film synthesis, and making stock Malachite green solutions. Description
PVA weight-1.0029g (Polymer only) PVA weight-1.0038g (Polymer and clay) Na montmorillonite-0.1105g
DataStock Solutions were prepared by Dr. Hartings
2. 1% wt. HCl Solution
3. 0.2M Sodium Bicarbonate Solution
Notes and calculations1.Malachite green solutions ppm (mass/mass)Stock solution ; 200ppm = 0.02%
The mL of stock solution needed = final mL x the solution concentration / stock solution concentration
Solution Calculations in the same order as the procedure
These standards were shared between Chem581, therefore some standards were made in larger amounts. A representative from each group help to prepare the standards. We (AMT) prepared the 0.20 ppm and 0.50 ppm Malachite Green Stock Solutions. Melvin, Becky, and Eleni prepared the 1.1 ppm and 1.4 ppm Malachite Green Stock Solutions. Madeleine and Alicia prepared the 1.7 ppm and 2.0 ppm Malachite Green Stock Solutions. See Madeleine's 8/29 Page for their calculations. James, Jake, and Mary prepared the 8.0 ppm, 80.0 ppm, and 200.0 ppm Malachite Green Stock Solutions. See James's 8/29 Page for their calculations.
2.Molar Absorptivity of Malachite GreenFigure 1. Shows the calibration curve for wavelength 617nm. Beer's Law explains that A= Ɛbc, where Ɛ = molar absorptivity in the units of cm-1*M-1, b = path length, and c = concentration. The molar absorptivity of the malachite green at 617nm is 0.1193. This is identified by translating Beer's Law to the slope of the calibration curve y=mx+b; where y = absorbance (y axis), x = concentration (x axis), and m = molar absorptivity. Figure 2. Shows the calibration curve for wavelength 423nm. Beer's Law explains that A= Ɛbc, where Ɛ = molar absorptivity in the units of cm-1*M-1, b = path length, and c = concentration. The molar absorptivity of the malachite green at 617nm is 0.1193. This is identified by translating Beer's Law to the slope of the calibration curve y=mx+b; where y = absorbance (y axis), x = concentration (x axis), and m = molar absorptivity. The standard curve for wavelength 423 has an line fit that is below acceptable values for calibration plots. This is due to the calibration point 1.1. |