User:TChan: Difference between revisions

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==W 6.14.06==
<div class="tabs-blue">
1.
<ul>
==Tu 6.13.06==
<li id="current">[[TChan|Project Overview]]</li>
1. Picked Colonies of Existing BioBrick Transformants
<li>[[TChan/Schematics|Schematics]]</li>
*Picked:
<li>[[TChan/OligoList|Oligo List]]</li>
  3 colonies of E7,
<li>[[TChan/Scaffold|Scaffold]]</li>
  2 colonies of E0,
<li>[[TChan/Images|Images]]</li>
  2 colonies of R0, and numbered accordingly
<li>[[TChan/iGEMNotes|iGEM Notes]]</li>
*Grew each colony in 5mL LB + 5uL of 50mg/mL Amp (1:100 dilution).
</ul>
</div>
<br style="clear:both">
<br>


2. Ran Gel of DNA Nanostructures
*See [[IGEM:Harvard/2006/DNA_nanostructures|iGEM 2006 Page]] for more info
*Ran 2% agarose gel of the nanostructure reaction and the 2 controls for ~40 min at 130V.  (NB: Only left four lanes are ours.)
  [[Image:Tc_mm_pt_lh_folding_0613.jpg|thumb|left|Nanostructure Gel]]
 
  1kb Ladder | Oligo + Scaffold | -Oligo | -Scaffold




==iGEM 2006 Notebook==
<calendar>
name=IGEM:Harvard/2006/DNA_nanostructures/Notebook
date=2006/07/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>


<calendar>
name=IGEM:Harvard/2006/DNA_nanostructures/Notebook
date=2006/10/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>






==Notebook==
<calendar>
name=TChan/Notebook
date=2006/2/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>


<calendar>
name=TChan/Notebook
date=2006/5/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>






==M 6.12.06==
1. Folded DNA Nanostructures Using Shawn's Pre-designed Oligo Staples and Scaffold ssDNA
*Three reactions below were mixed according to [http://openwetware.org/wiki/Folding_DNA_nanostructures  given protocol].
oligos + scaffold
-oligos
-scaffold
2. Transformed Bacteria with Existing BioBrick Plasmids
*Transformed each BioBrick plasmid below into OneShot Top10 competent cells (Invitrogen) according to the following protocol.
  R0010 (lac operon promoter),
  E7104 (T4 promoter + gfp),
  and E0241 (gfp).
===Top10 Transformation===
  Thaw cells; aliquot out ~30uL per transformation.
  Add desired amount of plasmid DNA (1 uL in this case?) to cells and leave on ice for 30 min.
  Heat shock at 37&degC for 30 sec in a heating block. 
  Ice for 2 min.
  Shake in 37%deg incubator for 1 hr.
  Pour onto plates with the required antibiotic (carbinomycin in this case); spread with beads or plate spreader.
  Incubate plates in 37%deg incubator overnight.
HOMEWORK:
#Past iGEM Projects Presentation: UCSF
#Prospective Projects Research:
  Lactobacillus Hijacking
  Receptor-based DNA Nanostructure Latch
  Biocryptography w/ DNA Nanostructures
  ?


==Contact Info==
==Contact Info==
/Tiffany Chan
  Tiffany Chan
/Cell: 781 330 1969
  Biochemical Sciences
/[mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]
  Kirkland '07
  Academic Interests: DNA nanostructures, non-coding RNAs (SO. HOT.), drug design and delivery
  [mailto:chan.tiffany@gmail.com chan.tiffany at gmail.com]
  Currently taking [http://openwetware.org/wiki/Harvard:Biophysics_101/2007 Biophysics 101]

Latest revision as of 13:12, 9 April 2009




iGEM 2006 Notebook

<calendar> name=IGEM:Harvard/2006/DNA_nanostructures/Notebook date=2006/07/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=IGEM:Harvard/2006/DNA_nanostructures/Notebook date=2006/10/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>


Notebook

<calendar> name=TChan/Notebook date=2006/2/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

<calendar> name=TChan/Notebook date=2006/5/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>



Contact Info

 Tiffany Chan
 Biochemical Sciences
 Kirkland '07
 Academic Interests: DNA nanostructures, non-coding RNAs (SO. HOT.), drug design and delivery
 chan.tiffany at gmail.com
 Currently taking Biophysics 101
Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:TChan&oldid=300657"