User:Sydney Marshall/Notebook/Protease Project/2015/09/23

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Objective To perform FTIR, DSC and XRD analysis on AuNP lysozyme fibers. Description The protocol for today can be found here. Analysis Prep 10 5mL Fiber samples were transferred into 5 15mL centrifuge tubes. Samples were then centrifuged for 5 mins at 4000 R. XRD Droplets of fibers were placed on a small amount of PlayDoh and kneaded together. The combination pf PlayDoh and fibers was placed into a lid specific for XRD. DSC Sample 1: AuNP Fiber Pan & Lid: 51.37 mg Sample: 3.49 mg Together: 53.73 mg Sample 2: AuNP Fiber Pan & Lid: 50.80 mg Sample: 4.43 mg Together: 53.74 mg Sample 3: AuNP Fiber Pan & Lid: 50.71 mg Sample: 3.63 mg Together: 54.44 mg Sample 4: Lysozyme Pan & Lid: 51.237 mg Sample: 3.04 mg Together: 54.38 mg Sample 1 was placed in well 27 in the DSC apparatus. Samples 2, 3 and 4 were placed in wells 28, 29 and 30, respectively. Cycling Settings: Equilibrate at 20°C Ramp up to 5°C/min and to 100°C. Isothermal - 3 mins Ramp down to 5°C/min and to 20°C Repeat (blue arrow) Lastly, external event (OFF) <= (only for the last sample was this configuration added.)