User:Sunny Sharma

Contact Info

• Sunny Sharma, PhD
• Email me through OpenWetWare

Education

• Postdoctoral Researcher at Department of Cell Biology and Neurosciences.
• Research Fellow at MGH Cancer Center– Harvard Medical School
• Research Associate at Institute of Molecular Biosciences, Goethe University, Frankfurt am Main, Germany [1]
• 2014, PhD (Biology), Goethe University, Frankfurt am Main, Germany [2]
• 2007, MS (Molecular Biology & Biochemistry), Guru Nanak Dev University, Amritsar, India [3]
• 2005, BS, Bangalore University, Bangalore, India [4]

Awards

TFMD (Tosteon and Fund for Medical Discovery) Fellowship. (2018) [5]

EMBO (European Molecular Biology Organisation) Long Term Fellowship. (2014-2016)[6]

DAAD (Deutsche Akademische Austauschdienst) PhD scholarship (2010 - 2014) [7]

CEF (Excellence Cluster-Frankfurt am Main) PhD fellowship (2009 - 2010) [8]

ICMR (Indian Council of Medical Research) Junior Research Fellowship (2009 - 2010) [9]

Research interests

1. Ribosome Biogenesis
2. RNA modifications
3. Developmental Biology
4. Immunology
5. Gerontology

Publications

20) Mammalian Nudix proteins cleave nucleotide metabolite caps on RNAs. [10]

19) Identification of the 3-amino-3-carboxypropyl (acp) transferase enzyme responsible for acp3U formation at position 47 in Escherichia coli tRNAs. [11]

18) A single N1-methyladenosine on the large ribosomal subunit rRNA impacts locally its structure and the translation of key metabolic enzymes. [12]

17) Identification of sites of 2′-O-methylation vulnerability in human ribosomal RNAs by systematic mapping.[13]

16) Specialized box C/D snoRNPs act as antisense guides to target RNA base acetylation.[14]

15) Mapping of Complete Set of Ribose and Base Modifications of Yeast rRNA by RP-HPLC and Mung Bean Nuclease Assay.[15]

14) Tuning the ribosome: the influence of rRNA modification on eukaryotic ribosome biogenesis and function.[16]

13) Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsible for 18S rRNA hypermodification in yeast and humans. [17]

12)‘View From A Bridge’: A New Perspective on Eukaryotic rRNA Base Modification.[18]

11) The reverse transcription signature of N-1-methyladenosine in RNA-Seq is sequence dependent.[19]

10) Yeast Kre33 and human NAT10 are conserved 18S rRNA cytosine acetyltransferases that modify tRNAs assisted by the adaptor Tan1/THUMPD1.[20]

9) Identification of a new ribose methylation in the 18S rRNA of S. cerevisiae.[21]

8) Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.[22]

7) Absolute and relative quantification of RNA modifications via biosynthetic isotopomers.[23]

6) Partial methylation at Am100 in 18S rRNA of baker´s yeast shows ribosome heterogeneity on the level of eukaryotic rRNA modification. Plos One [24]

5) Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae.[25]

4) Yeast Nop2 and Rcm1 methylate C2870 and C2278 of the 25S rRNA, respectively.[26]

3) Identification of a novel methyltransferase, Bmt2, responsible for the N-1-methyl-adenosine base modification of 25S rRNA in Saccharomyces cerevisiae.[27]

2) Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA.[28]

1) Association of P2X7 receptor +1513 (A-->C) polymorphism with tuberculosis in a Punjabi population.[29]

Conferences/ Workshops

1. Plenary talk at 10th Ribosome Synthesis meeting 19th-25th August, Brussels.[30]
2. Seminar at RNA club, 16th October 2013 [31]
3. Plenary talk at Yeast Meeting, Frankfurt 2013 [32]
4. Poster presentation at 18th Annual RNA meeting of the RNA society, Davos, Switzerland [33]
5. Seminar at RNA club, 26th September 2012 [34]
6. Poster presentation at 9th International Conference on Ribosome Synthesis, Banff, Canada [35]

Useful links

• http://www.muk.uni-frankfurt.de/53895688/033?
• http://www.yeastgenome.org/
• http://www.rnaclub.de
• http://people.biochem.umass.edu/fournierlab/3dmodmap/main.php
• http://modomics.genesilico.pl/
• http://www.rna.icmb.utexas.edu/
• http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
• http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index