User:Sung-Hye Grieco

Photo-synthetic bacteria set up in fermenter suite

Sung-Hye H. Grieco

• Suite Manager, Single Cell Fermenter Suite, Centre for Blood Research, University of British Columbia
• Contact info via E-mail
• Lab website:http://cbr.ubc.ca/fermenter.htm

I work in the Centre for Blood Research at University of British Columbia. I learned about OpenWetWare after I attended Pacific Rim Summit on Industrial Biotechnology and Bioenergy meeting at Vancouver in 2008 September and listened the inspiring plenary lecture by Dr. Endy Drew about Synthetic Biology titled "Can we make Biology Easy to Engineer?", and I've joined because I wanted to share protocols and ideas about sciences and discuss about daily questions for myself and others.. After I joined it, I also realized that I can use this OpenWetWare for live website for communicating with fermenter users for on-going project using OpenWetWare LabNotebook.

Education

• 2005, Post doctoral resesarch, University of British Columbia , Vancouver, BC, Canada
• 2003, Post doctoral resesarch, Harvard Medical School , Boston, MA, USA
• 2001, PhD. Bioengineering, Osaka University , Osaka, Japan
• 1997, MS. Biochemistry, Catholic Medical School , Seoul, Korea

Research interests

1. Small-scale fermentation optimization and process development:
   Provide time and cost efficient production tools using fermentation equipment.
2. Building up fermentation data base:
   Provide on-line database from fermentation conducted from our suite with detailed fermentation conditions for result download, invoices, tracking down previous data, and data comparison and easy analysis.
3. Sharing scientific information for trouble shooting and protocal exchanges:
   Also provide information about basic materials (media composition, characteristics of host strains), instructions on how to prepare bioreactors, conducting fermentation, and analysis of fermentation data and off-line data.

Publications

1. Hao Q, Hong SH, and Maret W. Lipid raft-dependent endocytosis of metallothionein in HepG2 cells. J Cell Physiol. 2007 Feb;210(2):428-35. DOI:10.1002/jcp.20874 |
2. Hong SH, Hao Q, and Maret W. Domain-specific fluorescence resonance energy transfer (FRET) sensors of metallothionein/thionein. Protein Eng Des Sel. 2005 Jun;18(6):255-63. DOI:10.1093/protein/gzi031 |
3. Hong SH and Maret W. A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein. Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2255-60. DOI:10.1073/pnas.0438005100 |
4. Hong S, Toyama M, Maret W, and Murooka Y. High yield expression and single step purification of human thionein/metallothionein. Protein Expr Purif. 2001 Feb;21(1):243-50. DOI:10.1006/prep.2000.1372 |
5. Hong SH, Gohya M, Ono H, Murakami H, Yamashita M, Hirayama N, and Murooka Y. Molecular design of novel metal-binding oligomeric human metallothioneins. Appl Microbiol Biotechnol. 2000 Jul;54(1):84-9. DOI:10.1007/s002530000360 |

Useful links

• Online Lab Notebook for Fermenter Users

• Introductory tutorial
• OpenWetWare help pages

1. User Guide (meta.wikimedia.org
2. Ask a Question about Editing-Related Questions
3. Advanced Editing (meta.wikimedia.org)
4. How to make wiki table (meta.wikimedia.org)
5. How to protect your page from editing by unauthorized users(mediawiki.org)
6. Magic Words(mediawiki.org)

Memo

1. How to delete page: after {{ sign, type [deleteme] and finish with }} sign.