User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/19: Difference between revisions
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added 35ul oil | added 35ul oil | ||
3min initial melt | 3min initial melt\ | ||
==2550 sample == | |||
Master mix | |||
*30ul F primer yes | |||
*30ul R primer yes | |||
*60ul dNTPs yes | |||
*3ul Taq (0.5u per tube) | |||
*75ul 10x buffer yes | |||
*45ul MgCl<sub>2</sub> yes | |||
*480ul H<sub>2</sub>O yes | |||
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Revision as of 02:29, 19 January 2013
 Project name
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Preliminary results generation
I will be doing a PCR with P2/P8 primers and then comparing against positive controls with ALL SAMPLES (except loris). Then I will repeat this with the 2550F primer pair at 55°C and comparing all these results. If I can't get consistency, I will order the NP MP primer set. Method: black pen - P2 - 60C blue - 2550F - 55C 29 samples
Tube
added 35ul oil 3min initial melt\ 2550 sampleMaster mix
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