User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 38: Line 38:
neg
neg


 
Results: no bands. Try lower annealing temperature - say 55°C


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 01:16, 18 January 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Results

  • last samples produced valid results when compared with positive controls

Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using.

Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples.

Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control

  • 1ul F primer
  • 1ul R primer
  • 2.0ul dNTPs
  • 0.5u Taq
  • 1ul DNA
  • 2.5ul 10x buffer
  • 1.5ul MgCl2
  • 16ul H2O
  • 1:1SWWBD
  • 2:2SWWBD
  • 3:R5#9
  • 4:R5#51
  • 5:R5#52
  • 6:R5#100
  • 7 :Pink Gold

male female neg

Results: no bands. Try lower annealing temperature - say 55°C


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Stuart_McKellar/Notebook/Bird_Sex_Testing/2013/01/15&oldid=668592"