User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15

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(Autocreate 2013/01/15 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing)
Current revision (04:16, 18 January 2013) (view source)
(Results)
 
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==Entry title==
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==Results==
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* Insert content here...
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* last samples produced valid results when compared with positive controls
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Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using.
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Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples.
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Master Mix:
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7 x lorikeets
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2 x positive controls (male female)
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1 negative control
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*1ul F primer
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*1ul R primer
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*2.0ul dNTPs
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*0.5u Taq
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*1ul DNA
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*2.5ul 10x buffer
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*1.5ul MgCl<sub>2</sub>
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*16ul H<sub>2</sub>O
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*1:1SWWBD
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*2:2SWWBD
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*3:R5#9
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*4:R5#51
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*5:R5#52
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*6:R5#100
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*7 :Pink Gold
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male
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female
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neg
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Results: no bands. Try lower annealing temperature - say 55°C
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Results

  • last samples produced valid results when compared with positive controls

Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using.

Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples.

Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control

  • 1ul F primer
  • 1ul R primer
  • 2.0ul dNTPs
  • 0.5u Taq
  • 1ul DNA
  • 2.5ul 10x buffer
  • 1.5ul MgCl2
  • 16ul H2O
  • 1:1SWWBD
  • 2:2SWWBD
  • 3:R5#9
  • 4:R5#51
  • 5:R5#52
  • 6:R5#100
  • 7 :Pink Gold

male female neg

Results: no bands. Try lower annealing temperature - say 55°C


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