User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09 - Revision history

Stuart McKellar: /* Bird PCR */

Stuart McKellar — Wed, 09 Jan 2013 00:25:12 GMT

Bird PCR

←Older revision		Revision as of 00:25, 9 January 2013
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	Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.		Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.

-	Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples.	+	Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples.

	Master Mix:		Master Mix:
Line 16:		Line 16:
	1 negative control		1 negative control

-	~~20ul total volume~~	+	*1ul F primer
		+	*1ul R primer
		+	*2.0ul dNTPs
		+	*0.5u Taq
		+	*1ul DNA
		+	*2.5ul 10x buffer
		+	*1.5ul MgCl<sub>2</sub>
		+	*16ul H<sub>2</sub>O

		+	*1:1SWWBD
		+	*2:2SWWBD
		+	*3:R5#9
		+	*4:R5#51
		+	*5:R5#52
		+	*6:R5#100
		+	*7 :Pink Gold
		+	male
		+	female
		+	neg

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Stuart McKellar: /* Entry title */

Stuart McKellar — Tue, 08 Jan 2013 23:06:14 GMT

Entry title

←Older revision		Revision as of 23:06, 8 January 2013
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Bird PCR==
-	* Insert content here...	+	Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers.
		+
		+	Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples.
		+
		+	Master Mix:
		+	7 x lorikeets
		+	2 x positive controls (male female)
		+	1 negative control
		+
		+	20ul total volume

Stuart McKellar: Autocreate 2013/01/09 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing

Stuart McKellar — Tue, 08 Jan 2013 22:53:47 GMT

Autocreate 2013/01/09 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__