User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09: Difference between revisions
From OpenWetWare
(Autocreate 2013/01/09 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing) |
|||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Bird PCR== | ||
Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. | |||
Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples. | |||
Master Mix: | |||
7 x lorikeets | |||
2 x positive controls (male female) | |||
1 negative control | |||
20ul total volume | |||
Revision as of 16:06, 8 January 2013
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Bird PCRAim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control 20ul total volume
| |
