User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09: Difference between revisions
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== | ==Bird PCR== | ||
Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. | |||
Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples. | |||
Master Mix: | |||
7 x lorikeets | |||
2 x positive controls (male female) | |||
1 negative control | |||
*1ul F primer | |||
*1ul R primer | |||
*2.0ul dNTPs | |||
*0.5u Taq | |||
*1ul DNA | |||
*2.5ul 10x buffer | |||
*1.5ul MgCl<sub>2</sub> | |||
*16ul H<sub>2</sub>O | |||
*1:1SWWBD | |||
*2:2SWWBD | |||
*3:R5#9 | |||
*4:R5#51 | |||
*5:R5#52 | |||
*6:R5#100 | |||
*7 :Pink Gold | |||
male | |||
female | |||
neg | |||
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Revision as of 17:25, 8 January 2013
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Bird PCRAim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control
male female neg | |
