User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09: Difference between revisions
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== | ==Bird PCR== | ||
Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. | |||
Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples. | |||
Master Mix: | |||
7 x lorikeets | |||
2 x positive controls (male female) | |||
1 negative control | |||
20ul total volume | |||
Revision as of 16:06, 8 January 2013
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Bird PCRAim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control 20ul total volume
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