User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/09
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< User:Stuart McKellar | Notebook | Bird Sex Testing | 2013 | 01(Difference between revisions)
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Current revision (20:25, 8 January 2013) (view source) (→Bird PCR) |
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Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. | Aim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. | ||
| - | Method: run a PCR @ 60°C and with 1ul primers. Visualise on a 1% gel. 10 samples. | + | Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples. |
Master Mix: | Master Mix: | ||
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1 negative control | 1 negative control | ||
| - | + | *1ul F primer | |
| + | *1ul R primer | ||
| + | *2.0ul dNTPs | ||
| + | *0.5u Taq | ||
| + | *1ul DNA | ||
| + | *2.5ul 10x buffer | ||
| + | *1.5ul MgCl<sub>2</sub> | ||
| + | *16ul H<sub>2</sub>O | ||
| + | *1:1SWWBD | ||
| + | *2:2SWWBD | ||
| + | *3:R5#9 | ||
| + | *4:R5#51 | ||
| + | *5:R5#52 | ||
| + | *6:R5#100 | ||
| + | *7 :Pink Gold | ||
| + | male | ||
| + | female | ||
| + | neg | ||
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Bird PCRAim: run a PCR and gel with respect to the new information gathered in the last entry. Also order more primers. Method: run a PCR @ 60°C and with 1ul P2/P8 primers. Visualise on a 1% gel. 10 samples. Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control
male female neg | |
