User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/04: Difference between revisions
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== | ==Reconciling information and thoughts after break== | ||
I did some reading and thinking and research during the xmas break. This is the result. | |||
=Housekeeping Genes= | |||
Finally found some housekeeping primers: | |||
18S-F908 18S a AGCGAAAGCATTTGCCAAGA 401 This study (SVB) | |||
18S-R1309 18S a AGTCTCGTTCGTTATCGGAATT This study (SVB) | |||
(from http://wwwnc.cdc.gov/eid/article/11/5/05-0211-t1.htm) | |||
=Temps and new primers= | |||
P2/P8 primers work best at 60C, confirming my lab work. | |||
(from http://www.academia.edu/1460726/Sex_Identification_of_Some_Pet_Birds_by_Polymerase_Chain_Reaction-Based_Methods ) | |||
also, this paper suggests using some new primers and was getting results very similar to my ones. Going to try and order NP an MP primers. See this paper for what a positive and negative result looks like using P2/P8 primers. | |||
Revision as of 17:54, 3 January 2013
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Reconciling information and thoughts after breakI did some reading and thinking and research during the xmas break. This is the result. Housekeeping GenesFinally found some housekeeping primers: 18S-F908 18S a AGCGAAAGCATTTGCCAAGA 401 This study (SVB) 18S-R1309 18S a AGTCTCGTTCGTTATCGGAATT This study (SVB) (from http://wwwnc.cdc.gov/eid/article/11/5/05-0211-t1.htm) Temps and new primersP2/P8 primers work best at 60C, confirming my lab work. (from http://www.academia.edu/1460726/Sex_Identification_of_Some_Pet_Birds_by_Polymerase_Chain_Reaction-Based_Methods ) also, this paper suggests using some new primers and was getting results very similar to my ones. Going to try and order NP an MP primers. See this paper for what a positive and negative result looks like using P2/P8 primers.
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