User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/04
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| - | == | + | ==PCR with 2550/2178 primer pair at 60°C== |
| - | + | Aim: see if these primers are better at sex differentiation than the p2/p8 ones | |
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| + | method: | ||
| + | same master mix as yesterday - one extra sample IZ - ethel | ||
| + | All others corresond to the same letters as yesterday | ||
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| + | NB the 94°C soak went for 8.30 instead of 2 mins. | ||
| + | |||
| + | Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C. | ||
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PCR with 2550/2178 primer pair at 60°CAim: see if these primers are better at sex differentiation than the p2/p8 ones method: same master mix as yesterday - one extra sample IZ - ethel All others corresond to the same letters as yesterday NB the 94°C soak went for 8.30 instead of 2 mins. Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C.
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