User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/04

From OpenWetWare

< User:Stuart McKellar | Notebook | Bird Sex Testing | 2012 | 12(Difference between revisions)
Jump to: navigation, search
(Entry title)
Current revision (23:54, 3 December 2012) (view source)
(Entry title)
 

Current revision

Project name Main project page
Previous entry      Next entry

PCR with 2550/2178 primer pair at 60°C

Aim: see if these primers are better at sex differentiation than the p2/p8 ones

method: same master mix as yesterday - one extra sample IZ - ethel All others corresond to the same letters as yesterday

NB the 94°C soak went for 8.30 instead of 2 mins.

Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C.





Personal tools