User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/04: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==PCR with 2550/2178 primer pair at 60°C== | ||
Aim: see if these primers are better at sex differentiation than the p2/p8 ones | |||
method: | |||
same master mix as yesterday - one extra sample IZ - ethel | |||
All others corresond to the same letters as yesterday | |||
NB the 94°C soak went for 8.30 instead of 2 mins. | |||
Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C. | |||
Latest revision as of 22:18, 26 September 2017
Project name | Main project page Previous entry Next entry |
PCR with 2550/2178 primer pair at 60°CAim: see if these primers are better at sex differentiation than the p2/p8 ones method: same master mix as yesterday - one extra sample IZ - ethel All others corresond to the same letters as yesterday NB the 94°C soak went for 8.30 instead of 2 mins. Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C.
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