User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/04

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(Autocreate 2012/12/04 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing)
Current revision (00:54, 4 December 2012) (view source)
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==Entry title==
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==PCR with 2550/2178 primer pair at 60°C==
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* Insert content here...
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Aim: see if these primers are better at sex differentiation than the p2/p8 ones
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method:
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same master mix as yesterday - one extra sample IZ - ethel
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All others corresond to the same letters as yesterday
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NB the 94°C soak went for 8.30 instead of 2 mins.
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Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C.
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PCR with 2550/2178 primer pair at 60°C

Aim: see if these primers are better at sex differentiation than the p2/p8 ones

method: same master mix as yesterday - one extra sample IZ - ethel All others corresond to the same letters as yesterday

NB the 94°C soak went for 8.30 instead of 2 mins.

Otherwise exactly the same as yesterday. The profile was user 77 file 81 - should be 60C.





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