User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/03: Difference between revisions
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(Autocreate 2012/12/03 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing) |
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== | ==Visualising lori's p2/p8 on 1% gel== | ||
Aim: differentiate between male and female loris using PCR product of p2/p8 primers and running on 1% gel. | |||
Method: Made up a 1% gel using the life technologies SYBR safe green dye. No dye was added, as the soln was already a 1:1 dilution of dye in TBE buffer. It was easy to make up and dissolved the agar readily. | |||
Results: Gels can be seen here: [http://imgur.com/a/5omku] | |||
It looks as though the samples A and B are male and that D is female, with the rest being ambiguous. | |||
*A:1SWWBD | |||
*B:2SWWBD | |||
*D:R5#51 | |||
Follwing exact same protocol as here: | |||
[http://openwetware.org/wiki/User:Stuart_McKellar/Notebook/Bird_Sex_Testing/2012/12/02](here) | |||
Except annealing temp is 60°C. | |||
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Revision as of 23:15, 2 December 2012
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Visualising lori's p2/p8 on 1% gelAim: differentiate between male and female loris using PCR product of p2/p8 primers and running on 1% gel. Method: Made up a 1% gel using the life technologies SYBR safe green dye. No dye was added, as the soln was already a 1:1 dilution of dye in TBE buffer. It was easy to make up and dissolved the agar readily. Results: Gels can be seen here: [1] It looks as though the samples A and B are male and that D is female, with the rest being ambiguous.
Follwing exact same protocol as here: [2](here) Except annealing temp is 60°C. | |
