User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/03

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(Autocreate 2012/12/03 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing)
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==Entry title==
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==Visualising lori's p2/p8 on 1% gel==
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* Insert content here...
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Aim: differentiate between male and female loris using PCR product of p2/p8 primers and running on 1% gel.
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Method: Made up a 1% gel using the life technologies SYBR safe green dye. No dye was added, as the soln was already a 1:1 dilution of dye in TBE buffer. It was easy to make up and dissolved the agar readily.
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Results: Gels can be seen here: [http://imgur.com/a/5omku]
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It looks as though the samples A and B are male and that D is female, with the rest being ambiguous.
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*A:1SWWBD
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*B:2SWWBD
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*D:R5#51
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Follwing exact same protocol as here:
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[http://openwetware.org/wiki/User:Stuart_McKellar/Notebook/Bird_Sex_Testing/2012/12/02](here)
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Except annealing temp is 60°C.
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Revision as of 02:15, 3 December 2012

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Visualising lori's p2/p8 on 1% gel

Aim: differentiate between male and female loris using PCR product of p2/p8 primers and running on 1% gel.

Method: Made up a 1% gel using the life technologies SYBR safe green dye. No dye was added, as the soln was already a 1:1 dilution of dye in TBE buffer. It was easy to make up and dissolved the agar readily.

Results: Gels can be seen here: [1]

It looks as though the samples A and B are male and that D is female, with the rest being ambiguous.

  • A:1SWWBD
  • B:2SWWBD
  • D:R5#51

Follwing exact same protocol as here: [2](here)

Except annealing temp is 60°C.


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