User:Stuart McKellar/Notebook/Bird Sex Testing/2012/12/01 - Revision history

Stuart McKellar: /* higher annealing temp */

2012-12-01T05:31:26Z

higher annealing temp

←Older revision		Revision as of 05:31, 1 December 2012
Line 39:		Line 39:
	total volume in each is 25μL.		total volume in each is 25μL.

-	PCR: 94°C for 2m30s, 35 cycles of: 95°C 30s, 65°C for 30s, 72°C for 30s; final elongation 72°C for 5 mins.	+	PCR: 94°C for 2m30s, 35 cycles of: 95°C 30s, 65°C for 30s, 72°C for 30s; final elongation 72°C for 5 mins( actually went 10 mins by accident).

	results to be determined via agar gel electrophoresis on 1% gel stained using gelgreen.		results to be determined via agar gel electrophoresis on 1% gel stained using gelgreen.

Stuart McKellar: /* Entry title */

2012-12-01T03:34:40Z

Entry title

←Older revision		Revision as of 03:34, 1 December 2012
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==higher annealing temp==
-	* ~~Insert content here~~...	+
		+	Aim: test the p2/p8 and 2550/2178 primers at 65°C.
		+
		+	Method:
		+
		+	Sample 1:
		+	*A6: p2/p8 primers
		+	*B6: 2550/2178 primers
		+
		+	Sample 2:
		+	*C6: p2/p8 primers
		+	*D6: 2550/2178 primers
		+
		+	Negative control - no DNA (1ul h2O, 2550/2178 primers.)
		+
		+	Master mix (5x):
		+
		+	*12.5μL buffer 10x
		+	*12.5μL dNTPS
		+	*7.5μL MgCl<sub>2</sub>
		+	*62.5 H<sub>2</sub>O
		+	*1μL Taq
		+
		+
		+
		+	*A6: p2/p8 primers (2.5μL each, 1μL DNA) + 18.5 Master Mix
		+	*B6: 2550/2178 primers (2.5μL each, 1μL DNA) + 18.5 Master Mix
		+	*C6: p2/p8 primers (2.5μL each, 1μL DNA) + 18.5 Master Mix
		+	*D6: 2550/2178 primers (2.5μL each, 1μL DNA) + 18.5 Master Mix
		+
		+	total volume in each is 25μL.
		+
		+	PCR: 94°C for 2m30s, 35 cycles of: 95°C 30s, 65°C for 30s, 72°C for 30s; final elongation 72°C for 5 mins.
		+
		+	results to be determined via agar gel electrophoresis on 1% gel stained using gelgreen.

Stuart McKellar: Autocreate 2012/12/01 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing

2012-12-01T02:32:12Z

Autocreate 2012/12/01 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__