User:Stuart McKellar/Notebook/Bird Sex Testing/2012/11/06
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(Autocreate 2012/11/06 Entry for User:Stuart_McKellar/Notebook/Bird_Sex_Testing) |
Current revision (07:44, 6 November 2012) (view source) (→Entry title) |
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| - | == | + | ==GelGreen Gel Electrophoresis== |
| - | * | + | I am performing an agar gel electrophoresis to try and visualise a DNA ladder. I am using an iorodeo gel box. Gel was prepared as follows: |
| + | *4g Agarose | ||
| + | *200mL 1 x NaOH/Borax buffer | ||
| + | |||
| + | Heated in microwave until melted. Added 20ul Gelgreen when solution had cooled slightly. Mixed thoroughly by agitation and with a pippette tip. Cast gel. Filled chambers with 1 x NaOH/Borax buffer. Ran at 60mA and 100V for 1 hour. | ||
| + | |||
| + | It worked, but after a while it wasn't separating well because the ladder was large and the gel was 2%. Visualisation was quite good with the filter on top. | ||
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GelGreen Gel ElectrophoresisI am performing an agar gel electrophoresis to try and visualise a DNA ladder. I am using an iorodeo gel box. Gel was prepared as follows:
Heated in microwave until melted. Added 20ul Gelgreen when solution had cooled slightly. Mixed thoroughly by agitation and with a pippette tip. Cast gel. Filled chambers with 1 x NaOH/Borax buffer. Ran at 60mA and 100V for 1 hour. It worked, but after a while it wasn't separating well because the ladder was large and the gel was 2%. Visualisation was quite good with the filter on top.
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