User:Stuart McKellar/Notebook/Bird Sex Testing/2012/09/08

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(Lab work for 8/9/2012)
(Begin Lab work)
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Carried out extraction according to protocol for tissue EDNA hispex kit.
Carried out extraction according to protocol for tissue EDNA hispex kit.
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During the addition of the first solutions (1a & 1b) I used the pipette tip to disturb the blood. This pulled some of the fibres out from the paper that the blood was on. I don't think that this should hurt the reaction.
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Revision as of 04:43, 8 September 2012

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Lab work for 8/9/2012

  • I am going to attempt the extraction of DNA from some samples using the EDNA extraction kit and protocol. If this is successful I will go on to run a PCR and a Gel.

I am using 4 samples of the Diamond Doves, as they are being caught again in 4 weeks so it is OK if they don't work.

Begin Lab work

Transferred samples to 0.5mL tubes:

  • 6AMC
  • L3#29
  • 8AMC
  • L3#21

Carried out extraction according to protocol for tissue EDNA hispex kit.

During the addition of the first solutions (1a & 1b) I used the pipette tip to disturb the blood. This pulled some of the fibres out from the paper that the blood was on. I don't think that this should hurt the reaction.




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