User:Steven J. Koch/MTC/April 7 2011: Difference between revisions
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< User:Steven J. Koch | MTC
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#* Andy's tubulin description: http://openwetware.org/wiki/User:Andy_Maloney/Kinesin_%26_Microtubule_Page#Tubulin | #* Andy's tubulin description: http://openwetware.org/wiki/User:Andy_Maloney/Kinesin_%26_Microtubule_Page#Tubulin | ||
# Design Experiment | # Design Experiment | ||
#* "Tweak" the tubulin polymerization solution--Andy will prepare it. Don't do same as someone else. | #* "Tweak" the tubulin polymerization solution--Andy will prepare it. Don't do same as someone else. | ||
#** A boring example: dilute the tubulin, expecting fewer or longer MTs. | |||
#** Try not to do something "crazy." | |||
#** We can't change temperature (at least not easily)...polymerization time is an option | |||
#* Due Tuesday April 12 -- you can email it to me. | #* Due Tuesday April 12 -- you can email it to me. | ||
# Perform Experiment | # Perform Experiment | ||
Revision as of 08:03, 7 April 2011
MTC April 7 2011
Molecular Motors
- Viral packaging motor
- Flagellar motor(s)
- Archaea, Prokaryotes, Eukaryotes
- Wikipedia flagellum article: http://en.wikipedia.org/wiki/Flagellum
- E. Coli swimming movie http://www.rowland.harvard.edu/labs/bacteria/showmovie.php?mov=swimming_ecoli
- Electron density: http://www.youtube.com/watch?v=vA0hw29Q26g
- D2O results (Berg et al. 2010)
- Archaea, Prokaryotes, Eukaryotes
- Dynein, Myosin, Kinesin,
- Famous kinesin animation: http://www.youtube.com/watch?v=4AnPVuzF7CA&feature=related
- ATP Synthase
- Microscopy: http://www.youtube.com/watch?v=oFgMTdVRi6I
- ATP Synthase animation: http://www.youtube.com/watch?v=J8lhPt6V-yM&feature=related
- From above: http://www.youtube.com/watch?v=H0RchC0FbYU&NR=1
- What happens if we drive the motor with force? Mendeley: Itoh, H., Takahashi, A., Adachi, K., Noji, H., Yasuda, R., Yoshida, M., et al. (2004). Mechanically driven ATP synthesis by F1-ATPase. Nature, 427(6973), 465-468. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14749837.
- Polymerases / exonucleases / helicases / topoisomerases / ...
- RNA, DNA Polymerase reminder
- Ribosome wikipedia: http://en.wikipedia.org/wiki/Ribosome
- DNA Repair claymation: http://www.youtube.com/watch?v=9PEdqBuDMHM
- Helicase
- http://www.youtube.com/watch?v=UWNhwceMjfk
- Dan Johnson single-molecule helicase
- Topoisomerase
- Animation: http://www.youtube.com/watch?v=EYGrElVyHnU
- Keir Neuman experiment
- RNA, DNA Polymerase reminder
Microtubules field trip / HW--With Andy Maloney
- Read up on Tubulin
- Andy's tubulin description: http://openwetware.org/wiki/User:Andy_Maloney/Kinesin_%26_Microtubule_Page#Tubulin
- Design Experiment
- "Tweak" the tubulin polymerization solution--Andy will prepare it. Don't do same as someone else.
- A boring example: dilute the tubulin, expecting fewer or longer MTs.
- Try not to do something "crazy."
- We can't change temperature (at least not easily)...polymerization time is an option
- Due Tuesday April 12 -- you can email it to me.
- "Tweak" the tubulin polymerization solution--Andy will prepare it. Don't do same as someone else.
- Perform Experiment
- Thursday, April 14 9:30 AM At the CHTM, Don't Be Late!
- Analyze data
- Observations, make figure(s)
- Upload at least one to figshare.com (requires linking to your Science 3.0 account)
