User:Steven J. Koch/MTC/April 7 2011: Difference between revisions

Revision as of 08:01, 7 April 2011

MTC April 7 2011

Viral packaging motor
- http://www.youtube.com/watch?v=xiabW9txQqc&NR=1
Flagellar motor(s)
- Archaea, Prokaryotes, Eukaryotes
  - Wikipedia flagellum article: http://en.wikipedia.org/wiki/Flagellum
  - E. Coli swimming movie http://www.rowland.harvard.edu/labs/bacteria/showmovie.php?mov=swimming_ecoli
  - Electron density: http://www.youtube.com/watch?v=vA0hw29Q26g
  - D2O results (Berg et al. 2010)
Dynein, Myosin, Kinesin,
- Famous kinesin animation: http://www.youtube.com/watch?v=4AnPVuzF7CA&feature=related
ATP Synthase
- Microscopy: http://www.youtube.com/watch?v=oFgMTdVRi6I
- ATP Synthase animation: http://www.youtube.com/watch?v=J8lhPt6V-yM&feature=related
  - From above: http://www.youtube.com/watch?v=H0RchC0FbYU&NR=1
- What happens if we drive the motor with force? Mendeley: Itoh, H., Takahashi, A., Adachi, K., Noji, H., Yasuda, R., Yoshida, M., et al. (2004). Mechanically driven ATP synthesis by F1-ATPase. Nature, 427(6973), 465-468. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=14749837.
Polymerases / exonucleases / helicases / topoisomerases / ...
- RNA, DNA Polymerase reminder
  - Ribosome wikipedia: http://en.wikipedia.org/wiki/Ribosome
- DNA Repair claymation: http://www.youtube.com/watch?v=9PEdqBuDMHM
- Helicase
  - http://www.youtube.com/watch?v=UWNhwceMjfk
  - Dan Johnson single-molecule helicase
- Topoisomerase
  - Animation: http://www.youtube.com/watch?v=EYGrElVyHnU
  - Keir Neuman experiment

Read up on Tubulin
- Andy's tubulin description: http://openwetware.org/wiki/User:Andy_Maloney/Kinesin_%26_Microtubule_Page#Tubulin
Design Experiment
- "Tweak" the tubulin polymerization solution--Andy will prepare it. Don't do same as someone else.
- Due Tuesday April 12 -- you can email it to me.
Perform Experiment
- Thursday, April 14 9:30 AM At the CHTM, Don't Be Late!
Analyze data
- Observations, make figure(s)
- Upload at least one to figshare.com (requires linking to your Science 3.0 account)

@@ Line 29: / Line 29: @@
 *** Keir Neuman experiment
-==Microtubules field trip / HW==
+==Microtubules field trip / HW--With Andy Maloney==
-* Design Experiment
+# Read up on Tubulin
-** "Tweak" the tubulin polymerization solution--Andy will prepare it.  Don't do same as someone else.
+#* Andy's tubulin description: http://openwetware.org/wiki/User:Andy_Maloney/Kinesin_%26_Microtubule_Page#Tubulin
-* Perform Experiment
+# Design Experiment
-** Thursday, April 14 9:30 AM '''At the CHTM, Don't Be Late!'''
+#* "Tweak" the tubulin polymerization solution--Andy will prepare it.  Don't do same as someone else.
-* Analyze data
+#* Due Tuesday April 12 -- you can email it to me.
-** Observations, make figure(s)
+# Perform Experiment
-** Upload at least one to figshare.com (requires linking to your Science 3.0 account)
+#* Thursday, April 14 9:30 AM '''At the CHTM, Don't Be Late!'''
+# Analyze data
+#* Observations, make figure(s)
+#* Upload at least one to figshare.com (requires linking to your Science 3.0 account)