User:Stevej: Difference between revisions
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{{protocols group|Nucleic acids|2= | {{protocols group|Nucleic acids|2= | ||
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Revision as of 15:06, 23 May 2007
Nucleic acids
RNA
- Agarose gel electrophoresis
- Endy:Northern Blot, 32P End-Labeled Probes
- RNA extraction
- Probe Prep, 32P End-Labeled Probes
- In vitro transcription with T7 RNA polymerase
- Phenol/chloroform extraction
- Endy:RNA extraction
- Ethanol precipitation of nucleic acids
- RNase Protection Assay
- Northern Blot, 32P End-Labeled Probes
- RNA Half-life
- Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing
- RNA Quality Control
- Affymetrix Target Hybridization
- Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing
- Knight:In vitro transcription
- RNA electrophoresis
- Knight:RNA electrophoresis/Denaturing
- Knight:RNA electrophoresis
- Knight:RNA electrophoresis/Native
- Etchevers:Extraction ARN francais
- Sauer:RNA Purification from E. coli
- Quantitating nucleic acids
- PCR techniques
- Real-time PCR
- QRT-PCR/Single tube
- QRT-PCR
- Alm:Agarose gel electrophoresis
- QRT-PCR/Two tubes
- Q-PCR
- RNA extraction using trizol/tri
- Endy:Northern blot, AlkPhos end-labeled probes
- Bioanalyzer
- RNA blot (Northern)
- Dahlquist:DNA Microarray Protocol
- Griffin:Nested RT-PCR
- DIYbio:Notebook/Keiki Gels
- Making RNA probes with T7 transcription
DNAse 1 Protocol
Routine Protocol (10ug RNA in a 50ul rxn)
