User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09

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(BSA and HAuCl4 Solutions)
(Gold:BSA ratios)
Line 23: Line 23:
**fixed: .25mM Au
**fixed: .25mM Au
**new BSA: 0.00417mM
**new BSA: 0.00417mM
-
**solution: 1mL 2.5mM Au + 0.278mL 15μM BSA + 10mL H<sub>2</sub>O
+
**solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H<sub>2</sub>O
*Ratio 2: 90 Au:BSA
*Ratio 2: 90 Au:BSA
**fixed: .25mM Au
**fixed: .25mM Au
**new BSA: 0.00278mM
**new BSA: 0.00278mM
-
**solution: 1mL 2.5mM Au + 0.185mL 15μM BSA + 10mL H<sub>2</sub>O
+
**solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H<sub>2</sub>O
*Ratio 3: 120 Au:BSA
*Ratio 3: 120 Au:BSA
**fixed: .25mM Au
**fixed: .25mM Au
**new BSA: 0.00208mM
**new BSA: 0.00208mM
-
**solution: 1mL 2.5mM Au + 0.139mL 15μM BSA + 10mL H<sub>2</sub>O
+
**solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H<sub>2</sub>O
*Ratio 4: 150 Au:BSA
*Ratio 4: 150 Au:BSA
**fixed: .25mM Au
**fixed: .25mM Au
**new BSA: 0.00167mM
**new BSA: 0.00167mM
-
**solution: 1mL 2.5mM Au + 0.111mL 15μM BSA + 10mL H<sub>2</sub>O
+
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H<sub>2</sub>O
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Revision as of 11:55, 9 July 2013

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BSA and HAuCl4 Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.01mg
      • therefore, I need 11.0048mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 20.120mg BSA
      • therefore, I need 20.0879mL of H2O

Gold:BSA ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
  • Ratio 2: 90 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O


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