User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(BSA and HAuCl4 Solutions)
(BSA and HAuCl4 Solutions)
Line 19: Line 19:
***therefore, I need 20.0879mL of H<sub>2</sub>O
***therefore, I need 20.0879mL of H<sub>2</sub>O
 +
==Gold:BSA ratios==
 +
*Ratio 1: 60 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00417mM
 +
**solution: 1mL 2.5mM Au + 0.278mL 15μM BSA + 10mL H<sub>2</sub>O
 +
*Ratio 2: 90 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00278mM
 +
**solution: 1mL 2.5mM Au + 0.185mL 15μM BSA + 10mL H<sub>2</sub>O
 +
*Ratio 3: 120 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00208mM
 +
**solution: 1mL 2.5mM Au + 0.139mL 15μM BSA + 10mL H<sub>2</sub>O
 +
*Ratio 4: 150 Au:BSA
 +
**fixed: .25mM Au
 +
**new BSA: 0.00167mM
 +
**solution: 1mL 2.5mM Au + 0.111mL 15μM BSA + 10mL H<sub>2</sub>O
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 11:24, 9 July 2013

Project name Main project page
Previous entry      Next entry

BSA and HAuCl4 Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.01mg
      • therefore, I need 11.0048mL H2O
  • BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 20.120mg BSA
      • therefore, I need 20.0879mL of H2O

Gold:BSA ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 1mL 2.5mM Au + 0.278mL 15μM BSA + 10mL H2O
  • Ratio 2: 90 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 1mL 2.5mM Au + 0.185mL 15μM BSA + 10mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 1mL 2.5mM Au + 0.139mL 15μM BSA + 10mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 1mL 2.5mM Au + 0.111mL 15μM BSA + 10mL H2O


Personal tools