User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/07/09

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(Gold:BSA ratios)
Current revision (08:39, 16 July 2013) (view source)
(BSA and HAuCl4 Solutions)
 
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Line 13: Line 13:
***actual amount used: 10.01mg
***actual amount used: 10.01mg
***therefore, I need 11.0048mL H<sub>2</sub>O
***therefore, I need 11.0048mL H<sub>2</sub>O
-
*BSA solution
+
*89-98,000 BSA solution
**MW = 66776g
**MW = 66776g
**in 20mL 15μM solution, I need 20.032mg BSA
**in 20mL 15μM solution, I need 20.032mg BSA
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**new BSA: 0.00167mM
**new BSA: 0.00167mM
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H<sub>2</sub>O
**solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H<sub>2</sub>O
 +
*All solutions put into the 80°C oven at 12:07PM and taken out at 4PM
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BSA and HAuCl4 Solutions

  • Au solution of HAuCl4:3H2O
    • MW = 393.83 g/mol
    • in 10mL 2.5mM solution, I need 9.096mg HAuCl4:3H2O
      • actual amount used: 10.01mg
      • therefore, I need 11.0048mL H2O
  • 89-98,000 BSA solution
    • MW = 66776g
    • in 20mL 15μM solution, I need 20.032mg BSA
      • actual amount used: 20.120mg BSA
      • therefore, I need 20.0879mL of H2O

Gold:BSA ratios

  • Ratio 1: 60 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00417mM
    • solution: 1mL 2.5mM Au + 2.78mL 15μM BSA + 6.22mL H2O
  • Ratio 2: 90 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00278mM
    • solution: 1mL 2.5mM Au + 1.85mL 15μM BSA + 7.15mL H2O
  • Ratio 3: 120 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00208mM
    • solution: 1mL 2.5mM Au + 1.39mL 15μM BSA + 7.61mL H2O
  • Ratio 4: 150 Au:BSA
    • fixed: .25mM Au
    • new BSA: 0.00167mM
    • solution: 1mL 2.5mM Au + 1.11mL 15μM BSA + 7.89mL H2O
  • All solutions put into the 80°C oven at 12:07PM and taken out at 4PM


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